Studies on the Metabolism of C19 Steroids in Rat Liver. 6alpha-Hydroxylation of 3-Oxo-Delta4-Steroids in Rat Liver Microsomes
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منابع مشابه
On the Mechanism of Drug Hydroxylation in Rat Liver Microsomes
The TPNH- and O(2)-dependent drug hydroxylation system of liver microsomes has been studied using normal rats and rats in which the drug-hydroxylating activity has been enhanced by repeated injections of phenobarbital. The oxidative demethylation of aminopyrine is employed as an assay. Optimal conditions for the assay with regard to the concentrations of TPNH and aminopyrine are established. TP...
متن کاملSex differences in cytochrome P-450-dependent 25-hydroxylation of C27-steroids and vitamin D3 in rat liver microsomes.
Monoclonal antibodies directed against the cytochrome P-450 catalyzing 25-hydroxylation of C27-steroids and vitamin D3 (Andersson, S., Holmberg, I., and Wikvall, K. (1983) J. Biol. Chem. 258, 6777-6781) have been prepared by immunization of mice in hind footpads. Lymph node cells from the mice were fused with the Sp 2/0-Ag 14 line of mouse myeloma cells. A monoclonal antibody, designated MAb-25...
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A constitutive cytochrome P-450 catalyzing 25-hydroxylation of C27-steroids and vitamin D3 was purified from rat liver microsomes. The enzyme fraction contained 16 nmol of cytochrome P-450/mg of protein and showed only one protein band with a minimum molecular weight of 51,000 upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified cytochrome P-450 catalyzed 25-hydroxylatio...
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In a study of the mechanism whereby 5a-bile acids are formed from cholestanol, the 7a-hydroxylation of cholestanol was investigated in rat liver preparations in vitro. I t was found that in the presence of NADPH and oxygen, rat liver microsomes catalyzed the 70-hydroxylation of cholestanol to the same extent as that of cholesterol. The rate of the hydroxylation was enhanced by prior treatment o...
متن کاملDigitoxin metabolism by rat liver microsomes.
published by admin on Fri, 03/14/2014 8:53am Title Digitoxin metabolism by rat liver microsomes. Publication Type Journal Article Year of Publication 1975 Authors Schmoldt, A, Benthe, HF, Haberland, G Journal Biochem Pharmacol Volume 24 Issue 17 Pagination 1639-41 Date Published 1975 Sep 1 ISSN 0006-2952
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb00860.x